Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Identifieur interne : 001670 ( Main/Exploration ); précédent : 001669; suivant : 001671Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Auteurs : Jian Lei [Allemagne] ; Jeroen R. Mesters [Allemagne] ; Christian Drosten [Allemagne] ; Stefan Anemüller [Allemagne] ; Qingjun Ma [Allemagne] ; Rolf Hilgenfeld [Allemagne]Source :
- Antiviral research [ 1872-9096 ] ; 2014.
Descripteurs français
- KwdFr :
- Alignement de séquences, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (enzymologie), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Cristallographie aux rayons X, Domaine catalytique, Données de séquences moléculaires, Modèles moléculaires, Papaïne (), Papaïne (antagonistes et inhibiteurs), Papaïne (génétique), Protéines virales (), Protéines virales (antagonistes et inhibiteurs), Protéines virales (génétique), Structure tertiaire des protéines, Séquence d'acides aminés.
- MESH :
- antagonistes et inhibiteurs : Papaïne, Protéines virales.
- enzymologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Papaïne, Protéines virales.
- Alignement de séquences, Coronavirus du syndrome respiratoire du Moyen-Orient, Cristallographie aux rayons X, Domaine catalytique, Données de séquences moléculaires, Modèles moléculaires, Papaïne, Protéines virales, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Catalytic Domain, Crystallography, X-Ray, Middle East Respiratory Syndrome Coronavirus (chemistry), Middle East Respiratory Syndrome Coronavirus (enzymology), Middle East Respiratory Syndrome Coronavirus (genetics), Models, Molecular, Molecular Sequence Data, Papain (antagonists & inhibitors), Papain (chemistry), Papain (genetics), Protein Structure, Tertiary, Sequence Alignment, Viral Proteins (antagonists & inhibitors), Viral Proteins (chemistry), Viral Proteins (genetics).
- MESH :
- chemical , antagonists & inhibitors : Papain, Viral Proteins.
- chemistry : Middle East Respiratory Syndrome Coronavirus, Papain, Viral Proteins.
- enzymology : Middle East Respiratory Syndrome Coronavirus.
- genetics : Middle East Respiratory Syndrome Coronavirus, Papain, Viral Proteins.
- Amino Acid Sequence, Catalytic Domain, Crystallography, X-Ray, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Alignment.
Abstract
The Middle-East Respiratory Syndrome coronavirus (MERS-CoV) causes severe acute pneumonia and renal failure. The MERS-CoV papain-like protease (PL(pro)) is a potential target for the development of antiviral drugs. To facilitate these efforts, we determined the three-dimensional structure of the enzyme by X-ray crystallography. The molecule consists of a ubiquitin-like domain and a catalytic core domain. The catalytic domain displays an extended right-hand fold with a zinc ribbon and embraces a solvent-exposed substrate-binding region. The overall structure of the MERS-CoV PL(pro) is similar to that of the corresponding SARS-CoV enzyme, but the architecture of the oxyanion hole and of the S3 as well as the S5 specificity sites differ from the latter. These differences are the likely reason for reduced in vitro peptide hydrolysis and deubiquitinating activities of the MERS-CoV PL(pro), compared to the homologous enzyme from the SARS coronavirus. Introduction of a side-chain capable of oxyanion stabilization through the Leu106Trp mutation greatly enhances the in vitro catalytic activity of the MERS-CoV PL(pro). The unique features observed in the crystal structure of the MERS-CoV PL(pro) should allow the design of antivirals that would not interfere with host ubiquitin-specific proteases.
DOI: 10.1016/j.antiviral.2014.06.011
PubMed: 24992731
Affiliations:
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Mesters</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF)</wicri:regionArea><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Virology, University of Bonn Medical School, 53127 Bonn, Germany; German Center for Infection Research (DZIF), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical School, 53127 Bonn, Germany; German Center for Infection Research (DZIF)</wicri:regionArea><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion></affiliation></author><author><name sortKey="Anemuller, Stefan" sort="Anemuller, Stefan" uniqKey="Anemuller S" first="Stefan" last="Anemüller">Stefan Anemüller</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF)</wicri:regionArea><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF), Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF)</wicri:regionArea><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion></affiliation></author><author><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF), Germany. Electronic address: hilgenfeld@biochem.uni-luebeck.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany; German Center for Infection Research (DZIF)</wicri:regionArea><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion><wicri:noRegion>Germany; German Center for Infection Research (DZIF)</wicri:noRegion></affiliation></author></analytic><series><title level="j">Antiviral research</title><idno type="eISSN">1872-9096</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Catalytic Domain</term><term>Crystallography, X-Ray</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (enzymology)</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Papain (antagonists & inhibitors)</term><term>Papain (chemistry)</term><term>Papain (genetics)</term><term>Protein Structure, Tertiary</term><term>Sequence Alignment</term><term>Viral Proteins (antagonists & inhibitors)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (enzymologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Cristallographie aux rayons X</term><term>Domaine catalytique</term><term>Données de séquences moléculaires</term><term>Modèles moléculaires</term><term>Papaïne ()</term><term>Papaïne (antagonistes et inhibiteurs)</term><term>Papaïne (génétique)</term><term>Protéines virales ()</term><term>Protéines virales (antagonistes et inhibiteurs)</term><term>Protéines virales (génétique)</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Papain</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Papaïne</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Papain</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term><term>Papain</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Papaïne</term><term>Protéines virales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Catalytic Domain</term><term>Crystallography, X-Ray</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Structure, Tertiary</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Cristallographie aux rayons X</term><term>Domaine catalytique</term><term>Données de séquences moléculaires</term><term>Modèles moléculaires</term><term>Papaïne</term><term>Protéines virales</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Middle-East Respiratory Syndrome coronavirus (MERS-CoV) causes severe acute pneumonia and renal failure. The MERS-CoV papain-like protease (PL(pro)) is a potential target for the development of antiviral drugs. To facilitate these efforts, we determined the three-dimensional structure of the enzyme by X-ray crystallography. The molecule consists of a ubiquitin-like domain and a catalytic core domain. The catalytic domain displays an extended right-hand fold with a zinc ribbon and embraces a solvent-exposed substrate-binding region. The overall structure of the MERS-CoV PL(pro) is similar to that of the corresponding SARS-CoV enzyme, but the architecture of the oxyanion hole and of the S3 as well as the S5 specificity sites differ from the latter. These differences are the likely reason for reduced in vitro peptide hydrolysis and deubiquitinating activities of the MERS-CoV PL(pro), compared to the homologous enzyme from the SARS coronavirus. Introduction of a side-chain capable of oxyanion stabilization through the Leu106Trp mutation greatly enhances the in vitro catalytic activity of the MERS-CoV PL(pro). The unique features observed in the crystal structure of the MERS-CoV PL(pro) should allow the design of antivirals that would not interfere with host ubiquitin-specific proteases. </div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Lei, Jian" sort="Lei, Jian" uniqKey="Lei J" first="Jian" last="Lei">Jian Lei</name></noRegion><name sortKey="Anemuller, Stefan" sort="Anemuller, Stefan" uniqKey="Anemuller S" first="Stefan" last="Anemüller">Stefan Anemüller</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name><name sortKey="Mesters, Jeroen R" sort="Mesters, Jeroen R" uniqKey="Mesters J" first="Jeroen R" last="Mesters">Jeroen R. Mesters</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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